During our long lasing interest in nuclear endosperm we have addressed a number of aspects, including the biochemistry of starch synthesis, cell biology, genetics, developmental biology as well as the transcriptomics of barley, rice, maize wheat and Arabidopsis. Highlights of our findings include the discovery of the cytosolic AGPase in barley endosperm, identification of the aleurone specific Ltp2 promoter, radial nuclear based microtubular cellularization of early nuclear endosperm, the DEK1 dependent positional signaling underlying aleurone cell formation, development of an in vitro organ culture system for maize endosperm and the asymmetric contribution of the A, B and D genomes to the unique properties of hexaploid bread wheat. endo1.png
Endosperm; developmental and molecular biology. 2007. Editor: O.-A-Olsen. 248 pp. Springer Verlag. ISBN: 978-3-540-71234-3 (Print) 978-3-540-71235-0 (Online).
endo2.pngBrown,R.C., Lemmon,B.E. and Olsen. O.-A. (1994). Endosperm Development in Barley: Microtubule lnvolvement in the Morphogenetic Pathway. The Plant Cell 6:1241.1252 endo3.pngPfeifer,M., Kugler,K.G., Sandve,S.R., Zhan,B., Rudi,H., Hvidsten,T.R., IWGS, Mayer,K.F.X. and Olsen,O.-A. (2014). Genome interplay in the grain transcriptome of hexaploid bread wheat. Science 345 no. 6194. DOI: 10.1126/science.1250091.

Selected publications

LA Kleczkowski, P Villand, J Preiss, OA Olsen (1993). Kinetic mechanism and regulation of ADP-glucose pyrophosphorylase from barley (Hordeum vulgare) leaves. Journal of Biological Chemistry 268 (9), 6228-6233.

Brown,R.C., Lemmon,B.E. and Olsen. O.-A. (1994). Endosperm Development in Barley: Microtubule lnvolvement in the Morphogenetic Pathway. The Plant Cell 6:1241.1252

Aalen,R.B., Opsahl-Ferstad, H.G., Linnestad,C. and Olsen, O.-A. (1994). Transcripts encodingan oleosin and a dormancy‐ related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds. The Plant Journal 5 (3), 385-396.

Kalla,R., Shimamoto,K., Potter, K., Nielsen,P.S., Linnestad,C. and Olsen, O.-A. (1994). The promoter of the barley aleurone‐ specific gene encoding a putative 7 kDa lipid transfer protein confers aleurone cell specific expression in transgenic rice. The Plant Journal 6 (6), 849-860.

Olsen,O.-A., Brown,R.C. and Lemmon, B.E. (1995). Pattern and process of wall formation in developing endosperm. BioEssays 17 (9), 803-812.

Doan, D.N.P., Linnestad,C. and Olsen, O.-A. (1996). Isolation of molecular markers from the barley endosperm coenocyte and the surrounding nucellus cell layers. Plant molecular biology 31 (4), 877-886.

Linnestad C, Doan DN, Brown RC, Lemmon BE, Meyer DJ, Jung R, Olsen OA. (1998). Nucellain, a barley homolog of the dicot vacuolar-processing protease, is localized in nucellar cell walls. Plant Physiol 118: 1169-80.

Doan, D.N.P., Rudi,H. and Olsen,O.-A. 1999. The allosterically unregulated isoform of ADPglucose pyrophospholrylase from barley endosperm is the most likely source of ADP-glucose incorporated into endosperm starch. Plant Phys., 121:965-975.

Brown,R.C., Lemmon,B.E., Hong,C. and Olsen, O.-A. 1999. Development of endosperm in Arabidopsis thaliana. Sex Plant Reproduction 12;32-42

Olsen,O.-A. (1998). Endosperm Developments. “In This Issues”; Editorial comment, The Plant Cell, 10 (4):485-488. Invited editorial comment

Morino K, Olsen OA, Shimamoto K. 1999. Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene. Plant J. 1999 17(3):275-85.

Olsen, O.-A. (2001). Endosperm development: cellularization and cell fate specification. Annu. Rev. of Plant Physiol. Plant Mol. Biol. 52:233-267

Thorbjørnsen,T., Villand, P., Denyer,K., Olsen, O.-A. and Smith, A. (2002). Distinct isoforms of ADPglucose pyrophosphorylase occur inside and outside the amyloplasts in barley endosperm. The Plant Journal 10 (2), 243-250.

Lid, S. E., Gruis, D., Jung, R., Lorentzen, J. A., Ananiev, E., Chamberlin, M., Niu, X., Meeley, R., Nichols, S. E., and Olsen, O.-A. (2002). The defective kernel 1 (dek1) gene required for aleurone cell development in the endosperm of maize grains encodes a membrane protein of the calpain gene superfamily. PNAS 99, 5460-5465.

Wang C, Barry JK, Min Z, Tordsen G, Rao AG, Olsen OA.(2003). The calpain domain of the maize DEK1 protein contains the conserved catalytic triad and functions as a cysteine proteinase. J.Biol Chem. 5;278(36):34467-74.

Shen B, Li C, Min Z, Meeley RB, Tarczynski MC, Olsen OA. (2003). sal1 determines the number of aleurone cell layers in maize endosperm and encodes a class E vacuolar sorting protein. PNAS 27;100(11):6552-7.

Olsen O-A (2004) Nuclear Endosperm Development in Cereals and Arabidopsis thaliana . The Plant Cell 16: S214-S227.

Gruis, F., Guo, H., Selinger, D., Tian,Q and Olsen, O.-A.(2006). Surface position, not signaling from surrounding

Darren (Fred) Gruis, Hena Guo, Qing Tian and Odd-Arne Olsen (2006). Endosperm Cell and Organ Culture. In Endosperm - Development and Molecular biology (O.-A.Olsen, ed.).

Olsen, O.-A. and Becraft, P.W. (2013). Endosperm development. In Seed genomics, Wiley-Blackwell Publishing, John Wiley & Sons, Hoboken, NJ USA. Ed. P.Becraft.

Olsen, O.-A. and Weschke, W. (2014). Genetic and molecular aspects of barley grain development. In Barley Chemistry and Technology, 2nd Edition, Eds. P.Shewry and S.E.Ullrich. AACC series of monographs on grain chemistry and technology.

Pfeifer,M., Kugler,K.G., Sandve,S.R., Zhan,B., Rudi,H., Hvidsten,T.R., IWGS, Mayer,K.F.X. and Olsen,O.-A. (2014). Genome interplay in the grain transcriptome of hexaploid bread wheat. Science 345 no. 6194. DOI: 10.1126/science.1250091.